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2004•Pharmaceutical Journal of Chinese People's Liberation ArmyRequires access

Determination of Ginsenoside Re in Xinmaikang Injection by HPLC

Yong Yuan

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Abstract

Aim A method was developed for determining ginsenoside Re in Xinmaikang injection by HPLC to control its quality.Methods A Hypersil C 18 column with a solvent system consisting of acetonitrile-0.05% phosphoric acid water solution(18∶82) was used,and samples were detected by UV spectrometry at 203nm.Results The calibration curve was linear within the range of 0.05～1.25mg·ml -1 for ginsenoside Re.The average recovery of ginsenoside Re was 99.6% (RSD=1.8%).Conclution The method is simple,accurate and reliable,and can be used to control the quality of this preparation.

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What this paper is about

Aim A method was developed for determining ginsenoside Re in Xinmaikang injection by HPLC to control its quality.Methods A Hypersil C 18 column with a solvent system consisting of acetonitrile-0.05% phosphoric acid water solution(18∶82) was used,and samples were detected by UV spectrometry at 203nm.Results The calibration curve was linear within the range of 0.05～1.25mg·ml -1 for ginsenoside Re.The average recovery of ginsenoside Re was 99.6% (RSD=1.8%).Conclution The method is simple,accurate and reliable,and can be used to control the quality of this preparation.

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Available abstract

Aim A method was developed for determining ginsenoside Re in Xinmaikang injection by HPLC to control its quality.Methods A Hypersil C 18 column with a solvent system consisting of acetonitrile-0.05% phosphoric acid water solution(18∶82) was used,and samples were detected by UV spectrometry at 203nm.Results The calibration curve was linear within the range of 0.05～1.25mg·ml -1 for ginsenoside Re.The average recovery of ginsenoside Re was 99.6% (RSD=1.8%).Conclution The method is simple,accurate and reliable,and can be used to control the quality of this preparation.

Key concepts: Chromatography, Phosphoric acid, Calibration curve, Ginsenoside Rg1, High-performance liquid chromatography, Chemistry, Ginsenoside, Acetonitrile

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