Simultaneous Determination of Rutin and Quercetin in Carthamus tinctorius by High-Performance Liquid Chromatography with Chemiluminescence Detection

A method using HPLC-CL linkage was developed for simultaneous determination of Rutin and Quercetin in Carthamus tinctorius, based on the strong sensitive chemiluminescence of the K3Fe(CN) 6 systems in alkaline medium. The separation was carried out on a Hypersil ODS column with a mobile phase of ethanol-0.01% triethylamine (2:1,V/V). The linear ranges for Rutin and Quercetin determinations were 2.0×10-7 g/mL～2.5×10-5 g/mL and 5.1×10-7 g/mL～1.3×10-4g/mL with a detection limit(3δ)of 1.20×10-8 g/mL and 2.5×10-8 g/mL. The relative standard deviation for 5.0×10-6 g/mL Rutin and Quercetin was 1.4% and 2.3% (n = 6). The method can be useful for the determination of Rutin and Quercetin in Carthamus tinctorius samples.