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2008•Journal of Southwest China Normal UniversityRequires access

Determination of Rutin and Quercetin in Rosa Laevigata by Reversed-phase High Performance Liquid Chromatography

Yan Liu

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Abstract

A reversed-phase high performance liquid chromatography (RP-HPLC) method for the separa- tion and dertermination of the rutin and quercetin was developed.The separation was carried out on a Shim-pack VP-ODS 200 mm×4.6 mm column with a mobile phase of CH_3OH-H_2O(60:40).The flow rate was 0.8 mL/min and the wavelength of detection was 260 nm.The method had good linear relation- ship within the range 0.03—15μg/mL(r=0.999 0)and 0.04—20μg/mL(r=0.999 4)of rutin and querce- tin,respectively.The detection limit was 12.6 ng/mL and 15.2 ng/mL for rutin and quercetin,respec- tively.This method was applied for separating and determining quercetin and rutin in Rosa Laevigata.

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What this paper is about

A reversed-phase high performance liquid chromatography (RP-HPLC) method for the separa- tion and dertermination of the rutin and quercetin was developed.The separation was carried out on a Shim-pack VP-ODS 200 mm×4.6 mm column with a mobile phase of CH_3OH-H_2O(60:40).The flow rate was 0.8 mL/min and the wavelength of detection was 260 nm.The method had good linear relation- ship within the range 0.03—15μg/mL(r=0.999 0)and 0.04—20μg/mL(r=0.999 4)of rutin and querce- tin,respectively.The detection limit was 12.6 ng/mL and 15.2 ng/mL for rutin and quercetin,respec- tively.This method was applied for separating and determining quercetin and rutin in Rosa Laevigata.

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Available abstract

A reversed-phase high performance liquid chromatography (RP-HPLC) method for the separa- tion and dertermination of the rutin and quercetin was developed.The separation was carried out on a Shim-pack VP-ODS 200 mm×4.6 mm column with a mobile phase of CH_3OH-H_2O(60:40).The flow rate was 0.8 mL/min and the wavelength of detection was 260 nm.The method had good linear relation- ship within the range 0.03—15μg/mL(r=0.999 0)and 0.04—20μg/mL(r=0.999 4)of rutin and querce- tin,respectively.The detection limit was 12.6 ng/mL and 15.2 ng/mL for rutin and quercetin,respec- tively.This method was applied for separating and determining quercetin and rutin in Rosa Laevigata.

Key concepts: Rutin, Quercetin, Chromatography, High-performance liquid chromatography, Chemistry, Detection limit, Linear range, Organic chemistry

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