Determination of the Contents of Rutin and Quercetin in Buckwheat from Different Sources

A new method for determination of rutin and quercetin contents in buckwheat was developed in this paper.The extracts from buckwheat by Ultrasonic extraction was analyzed on a Shimadzu ODS column(4.6 mm×150 mm,5 μm)with a mobile phase of acetonitrile-water(containing 2% glacial acetic acid) for gradient elution at 360nm.And the flow rate was 1.0 mL/min.The linear ranges of rutin and quercetin concentrations were found within 0.023～1.150 μg(r=0.9996) and 0.02～0.60 μg(r=0.9999),respectively.The average recoveryies of rutin and quercetin were 98.5% and 95.8%,respectively.The method was simple,accurate and showed good reproducibility,which was suitable for the determination of rutin and quercetin contents in buckwheat.