Study on Callus Induction and Plantlet Regeneration from Immature Embyro among Different Maize Inbreds

In this study,6 inbred lines,which had higher inducing rate of embryogenic callus from 49 maize inbred lines by callus induction of immature embyro among different maize inbreds,were screened out.The type II callus of K6-64,K6-39,DL283-1,were suitable asreceptor material for transgenic research because of bright yellow and granular and had strong ability of long-term subculture and regeneration.In addition,the factors during in vitro culture of maize immature embryos were analyzed.The results showed that 1.5-2.0mg/L 2,4-D could induce maize immature embryos to produce embryogenic callus more effectively.A certain concentration of 6-BA and KT were able to promote maize callus differentiation,and there was no significant difference.