Study on immature embryos regeneration of maize inbred lines

Total 12 maize inbreds were used as the explants,by using the isolated culture method,the effects of different plant growth regulators and genotypes on immature embryo regeneration were studied.The results showed that callus induction rate was 90.5% when the concentration of 2,4-D was 3 mg.L-1.There existed great differences among maize genotypes.Zheng58 showed the highest frequency of callus induction which was 96.3%.The minimum frequency of callus induction was observ-ed in the inbred line Dong46 which belonged to the local germplasm.Genotype was an important factor affecting the callus status,which would further affect the regeneration of callus and plantlets.BA 1.0 mg.L-1+IBA 0.5 mg.L-1 was more suitable for callus differentiation.