ANALYSIS OF FACTORS INFLUENCING REGENERATION RATE OF EMBRYOGENIC CALLUS FROM IMMATURE EMBRYO OF MAIZE

In order to evaluate the factors,which affect embryogenic callus regeneration rate,immature embryos of 3 maize inbred lines were used.The important parameters of immature embryo,such as the regeneration frequency,of embryogenic callus,cloning index and differentiation frequency,were analyzed. The results revealed that differentiation frequency differed significantly among the 3 maize inbred lines.The embryogenic callus induction rate of 975-12 was affected notably compared to 416051 and 18-599 inbred lines under different environment conditions.The highest embryogenic callus induction of 18-599 and 416051 inbred lines were achieved as the immature embryo size was up to 2 mm,while that 2.5 mm of 975-12 is preferred.As to cloning index and differentiation rate,the highest finger could be got at the third or fourth generation.Collectively,18-599 and 416051 inbred lines had high embryogenic callus induction rate,strong cloning ability and easily differentiation,which made them as the candidates elite inbred lines for maize transformation.