Isolation,Purification,Determination of Relative Molecular Mass and Content in Polysaccharide from Aloe barbadensis Miller

Objective To isolate and purify the polysaccharide from Aloe barbadensis,determine relative molecular mass,total sugar content and protein content of the purified fraction(PSA2).Methods To adopt the method of water lifts and the alcohol immerse to extract the rude polysaccharides from Aloe barbadensis Miller.Rude polysaccharide was deproteinized by repeated freeze-thaw method,purified by DEAE–cellulose 52 and Sephadex G-100 columns,and the purified polysaccharide was termed as PSA2.HPGPC was used to determined the molecular weight of PSA2,the contents of the total soluble sugar and protein of PSA2 were determined by the phenol-sulfuric acid method and coomassie brilliant blue method,respectively.Results The HPGPC showed relative molecular mass of PSA2 was 8 457.4.The contents of total sugar and protein were 61.0% and 0.7%,respectively.Conclusion The extraction technology is simple and feasible,the HPGPC,phenol-sulfuric acid and coomassie brilliant blue are precise and rapid.