ScholarLens Back to search
2005•The Chinese Journal of Modern Applied PharmacyRequires access

Isolation,purification and determination of the polysaccharide of Aloe barbadensis

Hongbin Luo

Open publisher page 0 citations

Abstract

OBJECTIVE To isolate, purify the polysac ch aride of Aloe barbadensis Miller and establish the content determination method.METHOD The polysaccharide was precipitated with ethanol and fur ther purified on Sephadex G-100 column. Its total soluble sugar content was det ected colorimetrically by the phenol-sulfuric acid method with mannose as stand ard. RESULTS The total sugar content of polysaccharide I was 81.11%. The average recovery was 100.3%. CONCLUSION It is possible to extract and isolate purified polys accharide from Aloe barbadensis Miller. And the determination method is simple, rapid and well reproducible.

About this research paper

What this paper is about

OBJECTIVE To isolate, purify the polysac ch aride of Aloe barbadensis Miller and establish the content determination method.METHOD The polysaccharide was precipitated with ethanol and fur ther purified on Sephadex G-100 column. Its total soluble sugar content was det ected colorimetrically by the phenol-sulfuric acid method with mannose as stand ard. RESULTS The total sugar content of polysaccharide I was 81.11%. The average recovery was 100.3%. CONCLUSION It is possible to extract and isolate purified polys accharide from Aloe barbadensis Miller. And the determination method is simple, rapid and well reproducible.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

OBJECTIVE To isolate, purify the polysac ch aride of Aloe barbadensis Miller and establish the content determination method.METHOD The polysaccharide was precipitated with ethanol and fur ther purified on Sephadex G-100 column. Its total soluble sugar content was det ected colorimetrically by the phenol-sulfuric acid method with mannose as stand ard. RESULTS The total sugar content of polysaccharide I was 81.11%. The average recovery was 100.3%. CONCLUSION It is possible to extract and isolate purified polys accharide from Aloe barbadensis Miller. And the determination method is simple, rapid and well reproducible.

Key concepts: Polysaccharide, Chromatography, Sugar, Chemistry, Sephadex, Content determination, Mannose, Biochemistry

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Isolation,purification and determination of the polysaccharide of Aloe barbadensis — Research Paper | ScholarLens