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Determination of puerarin in gegentang granule by HPLC.

Zhao ChangJun, Hongming Liu

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Abstract

Objective To establish a RP-HPLC method for detecting puerarin in Gegentang Granule.Methods A C18 chromatographic column(250 mm × 4.6 mm,5μm) was used with the mobile phase consisting of acetonitrilemethanol-water(10:8:82).The detection wavelength was 250 nm.Results There was a good linear relationship in the concentration range of 4.008～200.4 μg/mL of puerarin,y = 0.012 6 x + 0.014 1(r = 1.000 0,n = 6).The average recovery was 100.02 % with RSD of 0.1 %.Conclusion The method is simple,accurate,and can be used for the quality control of Gegentang Granule.

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What this paper is about

Objective To establish a RP-HPLC method for detecting puerarin in Gegentang Granule.Methods A C18 chromatographic column(250 mm × 4.6 mm,5μm) was used with the mobile phase consisting of acetonitrilemethanol-water(10:8:82).The detection wavelength was 250 nm.Results There was a good linear relationship in the concentration range of 4.008～200.4 μg/mL of puerarin,y = 0.012 6 x + 0.014 1(r = 1.000 0,n = 6).The average recovery was 100.02 % with RSD of 0.1 %.Conclusion The method is simple,accurate,and can be used for the quality control of Gegentang Granule.

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Available abstract

Objective To establish a RP-HPLC method for detecting puerarin in Gegentang Granule.Methods A C18 chromatographic column(250 mm × 4.6 mm,5μm) was used with the mobile phase consisting of acetonitrilemethanol-water(10:8:82).The detection wavelength was 250 nm.Results There was a good linear relationship in the concentration range of 4.008～200.4 μg/mL of puerarin,y = 0.012 6 x + 0.014 1(r = 1.000 0,n = 6).The average recovery was 100.02 % with RSD of 0.1 %.Conclusion The method is simple,accurate,and can be used for the quality control of Gegentang Granule.

Key concepts: Puerarin, Chromatography, Granule (geology), High-performance liquid chromatography, Chemistry, Linear range, Linear relationship, Materials science

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