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2013•Journal of Yunnan University of Traditional Chinese MedicineRequires access

Determination of Puerarin in Biaoxu Ganmao Granule by HPLC

Yang Hai-l

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Abstract

Objective To establish a HPLC method for the determination of puerarin in Biaoxu Ganmao Granule. Methods The HPLC system consisted of the Phenomenex LUNA-C18(4.6mm×250mm,5μm)column,the mobile phase consisted of MeOH(21)-H2 O(79),the flow rate was 1.0mL·min-1,the column temperature was 30℃,The UV detector was set at 250nm. Results The linear response range was 0.023~0.460mg/ml(r=0.9999). The average recovery of puerarin was 99.1%,and RSD1.52%. Conclusion The method is simple,rapid,accurate and repeatable. It can be applied in determination of Puerarin in Biaoxu Ganmao Granule.

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Objective To establish a HPLC method for the determination of puerarin in Biaoxu Ganmao Granule. Methods The HPLC system consisted of the Phenomenex LUNA-C18(4.6mm×250mm,5μm)column,the mobile phase consisted of MeOH(21)-H2 O(79),the flow rate was 1.0mL·min-1,the column temperature was 30℃,The UV detector was set at 250nm. Results The linear response range was 0.023~0.460mg/ml(r=0.9999). The average recovery of puerarin was 99.1%,and RSD1.52%. Conclusion The method is simple,rapid,accurate and repeatable. It can be applied in determination of Puerarin in Biaoxu Ganmao Granule.

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Available abstract

Objective To establish a HPLC method for the determination of puerarin in Biaoxu Ganmao Granule. Methods The HPLC system consisted of the Phenomenex LUNA-C18(4.6mm×250mm,5μm)column,the mobile phase consisted of MeOH(21)-H2 O(79),the flow rate was 1.0mL·min-1,the column temperature was 30℃,The UV detector was set at 250nm. Results The linear response range was 0.023~0.460mg/ml(r=0.9999). The average recovery of puerarin was 99.1%,and RSD1.52%. Conclusion The method is simple,rapid,accurate and repeatable. It can be applied in determination of Puerarin in Biaoxu Ganmao Granule.

Key concepts: Puerarin, Chromatography, Granule (geology), Chemistry, High-performance liquid chromatography, Linear range, Materials science, Detection limit

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