2008•Research and Practice on Chinese MedicinesRequires access

Determination of Puerarin in Wuzhidixian Jinsui Gao by HPLC

Chang Yeon Yu, Nsn Natural

Abstract

Objective To establish a method to determine the content of Puerarin in Wuzhidixian Jinsui Gao.Methods HPLC was used to determine the content of Puerarin with detection wavelength at 250 nm.The mobile phase consists of a mixture of methanol-water(25:75).The flow rate was 0.8mL/min.Results The linear regression for Puerarin was obtained at the range of 3~48 μg/mL.The average recovery of Puerarin were 99.0%,RSD were 2.1%(n=6).Conclusion The method is sensitive,accurate,reproducible and exclusive.It can be used for quality control of Wuzhidixian Jinsui Gao.

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Available abstract

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Linear range, Chemistry, Methanol, Recovery rate, Medicine