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2014•Lishizhen Medicine and Materia Medica ResearchRequires access

Determination of Puerarin in Cangling Zhixie Oral Solution by HPLC

Xiaomi An

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Abstract

Objective To establish an HPLC method for determination of Puerarin in Cangling Zhixie oral solution. Methods The Innovation Explorer C18 HPLC Column was used with the mobile phase consisted of methanol- water( 22: 78); the flow rate was 1. 0 mL·min- 1; the detection wavelength was at 250 nm,and the column temperature was at 30 ℃. Results A good linear correlation of was observed within the range of 0. 2865 μg ~ 2. 865 μg( r = 0. 9999). The average recovery was 101. 89% with RSD of 2. 73%( n = 6). Conclusion The method is simple,rapid,accurate and reliable. It can be used for the determination of Puerarin in Cangling Zhixie oral solution.

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What this paper is about

Objective To establish an HPLC method for determination of Puerarin in Cangling Zhixie oral solution. Methods The Innovation Explorer C18 HPLC Column was used with the mobile phase consisted of methanol- water( 22: 78); the flow rate was 1. 0 mL·min- 1; the detection wavelength was at 250 nm,and the column temperature was at 30 ℃. Results A good linear correlation of was observed within the range of 0. 2865 μg ~ 2. 865 μg( r = 0. 9999). The average recovery was 101. 89% with RSD of 2. 73%( n = 6). Conclusion The method is simple,rapid,accurate and reliable. It can be used for the determination of Puerarin in Cangling Zhixie oral solution.

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Available abstract

Objective To establish an HPLC method for determination of Puerarin in Cangling Zhixie oral solution. Methods The Innovation Explorer C18 HPLC Column was used with the mobile phase consisted of methanol- water( 22: 78); the flow rate was 1. 0 mL·min- 1; the detection wavelength was at 250 nm,and the column temperature was at 30 ℃. Results A good linear correlation of was observed within the range of 0. 2865 μg ~ 2. 865 μg( r = 0. 9999). The average recovery was 101. 89% with RSD of 2. 73%( n = 6). Conclusion The method is simple,rapid,accurate and reliable. It can be used for the determination of Puerarin in Cangling Zhixie oral solution.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Chemistry, Linear range, Linear correlation, Linear relationship, Methanol

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