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Determination of puerarin in cangling zhixie oral solution by HPLC

Xian Li

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Abstract

Objective To establish an HPLC method for determination of puerarin in cangling zhixie oral solution.Methods The Agilent Eclipse XDB-C18(4.6 mm×150 mm,5 μm) was used,the mobile phase consisted of methanol-water(25:75),and the flow rate was 1.0 mL/min,the detective wavelength was set at 250 nm,the column temperature was at 30 ℃.Results The puerarin peak was well separated with other peaks,and a good linear correlation was observed within the range of 0.020 64~0.412 8 μg(r=0.999 9).The average recovery was 99.78% with RSD of 2.11%(n=6).Conclusion The method is rapid and simple,it can be used for the quality control of cangling zhixie oral solution with good selectivity,sensitivity and repeatability.

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Objective To establish an HPLC method for determination of puerarin in cangling zhixie oral solution.Methods The Agilent Eclipse XDB-C18(4.6 mm×150 mm,5 μm) was used,the mobile phase consisted of methanol-water(25:75),and the flow rate was 1.0 mL/min,the detective wavelength was set at 250 nm,the column temperature was at 30 ℃.Results The puerarin peak was well separated with other peaks,and a good linear correlation was observed within the range of 0.020 64~0.412 8 μg(r=0.999 9).The average recovery was 99.78% with RSD of 2.11%(n=6).Conclusion The method is rapid and simple,it can be used for the quality control of cangling zhixie oral solution with good selectivity,sensitivity and repeatability.

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Available abstract

Objective To establish an HPLC method for determination of puerarin in cangling zhixie oral solution.Methods The Agilent Eclipse XDB-C18(4.6 mm×150 mm,5 μm) was used,the mobile phase consisted of methanol-water(25:75),and the flow rate was 1.0 mL/min,the detective wavelength was set at 250 nm,the column temperature was at 30 ℃.Results The puerarin peak was well separated with other peaks,and a good linear correlation was observed within the range of 0.020 64~0.412 8 μg(r=0.999 9).The average recovery was 99.78% with RSD of 2.11%(n=6).Conclusion The method is rapid and simple,it can be used for the quality control of cangling zhixie oral solution with good selectivity,sensitivity and repeatability.

Key concepts: Puerarin, Repeatability, Chromatography, High-performance liquid chromatography, Linear range, Chemistry, Analytical Chemistry (journal), Detection limit

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