Synergistic effect of eicosapentaenoic acid and cisplatin on A-549 human lung cancer cell line

Objective To observe the effects of anti-proliferation and inducing apoptosis on A-549 cell line induced by eicosapentaenoic acid(EPA)and its synergistic effect with cisplatin(DDP).Methods A-549 cell line was cultured in vitro.A-549 cells were divided into group A and group B,the group B was divided into EPA 15(B1 group),30(B2 group),60(B3 group),DDP 30 μg/ml(B4 group),DDP30 μg/ml +EPA 15 μg/ml(B5 group),DDP 30 μg/ml +EPA 30 μg/ml(B6 group) and DDP 30 μg/ml+EPA 60 μg/ml(B7 group).The group A added control fluid 500 μl to replace the drugs,the experiment steps were alike.MTT assay was used to determine the effect of EPA on A-549 cells growth inhibitory.Morphological changes of the apoptosis of A-549 cells were observed by using inverted microscope.TUNEL was used to detect cell apoptotic index of A-549.Results ①in the group B1～B7,the growth of A-549 cell were inhibited and in dose and time dependent manner.EPA combined with DDP shows significant synergistic anti-tumor effect on A-549 cells.②The A-549 cells in group B showed morphological changes such as cellular volume decreased and become round,nuclear chromatin condensed,plasma membrance bleb formed,also the apoptotic body were observed by microscope nuclear chromatin condensation,loose between the cells and adherent ability decrease under invert microscope.③The apoptotic index(AI) of A-549 cells in group B1～B3 was 5.57%±0.82%,11.68%±1.26%,24.53%±1.68%,respectively,there was statistic significance compared with different concentration(P0.01).Conclusions EPA inhibited growth of A-549 cell line in vitro in dose and time dependent manner,EPA combined with DDP shows significant synergistic anti-tumor effect on A-549 cell line.