ScholarLens Back to search
2009•Journal of Hepatobiliary SurgeryRequires access

EFFECTS FO EICOSAPENTAENOIC ACID ON PROLIFERATION OF HEPG2 CELLS

Jingyu Cao

Open publisher page 0 citations

Abstract

Objective To study the effets of eicosapentaenoic acid (EPA)on the proliferation of HepG2 cells.Methods In,the EPA was added in the culture for the HepG2 cells.The proliferation of HepG2 cells was detected by MTT method,and the cell configuration was evaluated by fluorescent staining and transmission electron microspe,and the apoptosis was detected by flow cytometry (FCM).Results Compared with control group,the inhibition ration of the HepG2 cells in concentrations of EPA 45 μg/ml and 60 μg/ml is 36.7% and 52.1%,68.1% and 94.8%,94.2% and 99.7% on the 24 h,48 h and 72.An increasing of apoptosis on the test group was found by fluorescent staining,TEM,and FCM.Conclusions The HepG2 cells were inhibited by EPA and its effect depended on time and concentration of EPA.The phenomenon may be related with the apoptosis.

About this research paper

What this paper is about

Objective To study the effets of eicosapentaenoic acid (EPA)on the proliferation of HepG2 cells.Methods In,the EPA was added in the culture for the HepG2 cells.The proliferation of HepG2 cells was detected by MTT method,and the cell configuration was evaluated by fluorescent staining and transmission electron microspe,and the apoptosis was detected by flow cytometry (FCM).Results Compared with control group,the inhibition ration of the HepG2 cells in concentrations of EPA 45 μg/ml and 60 μg/ml is 36.7% and 52.1%,68.1% and 94.8%,94.2% and 99.7% on the 24 h,48 h and 72.An increasing of apoptosis on the test group was found by fluorescent staining,TEM,and FCM.Conclusions The HepG2 cells were inhibited by EPA and its effect depended on time and concentration of EPA.The phenomenon may be related with the apoptosis.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Objective To study the effets of eicosapentaenoic acid (EPA)on the proliferation of HepG2 cells.Methods In,the EPA was added in the culture for the HepG2 cells.The proliferation of HepG2 cells was detected by MTT method,and the cell configuration was evaluated by fluorescent staining and transmission electron microspe,and the apoptosis was detected by flow cytometry (FCM).Results Compared with control group,the inhibition ration of the HepG2 cells in concentrations of EPA 45 μg/ml and 60 μg/ml is 36.7% and 52.1%,68.1% and 94.8%,94.2% and 99.7% on the 24 h,48 h and 72.An increasing of apoptosis on the test group was found by fluorescent staining,TEM,and FCM.Conclusions The HepG2 cells were inhibited by EPA and its effect depended on time and concentration of EPA.The phenomenon may be related with the apoptosis.

Key concepts: Eicosapentaenoic acid, Apoptosis, Flow cytometry, Fluorescent staining, Staining, Cell growth, Medicine, Molecular biology

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
EFFECTS FO EICOSAPENTAENOIC ACID ON PROLIFERATION OF HEPG2 CELLS — Research Paper | ScholarLens