Mechanisms of DDP inhibiting cell migration in SKOV3 cells induced by extraneous IL-8

Objective To study the effect of exogenous human interleukin-8 on human ovarian cancer SKOV-3 cell migration and the intervention role of cisplatin on it; to preliminary explore the mechanisms of DDP inhibiting cell migration.Methods SKOV-3 cells were treated with DDP.Optimal concentration and effect time of DDP was determined with MTT assay.Transwell assay were used to study the intervention effect of DDP.Western blot assay was applied to detect NF-κB protein expression levels.Results MTT assay: compared with the control group, the best concentration of DDP which had a significant inhibitory effect on cell proliferation was 100 μg / ml( P 0.05).The concentration of DDP in the range of 200 μg / ml to 400 μg / ml also had the intervention effect,but cell growth inhibition rate between these groups showed no significant difference( P 0.05).DDP( 100 μg / ml) were treated on SKOV-3 cells 24 h,48 h,72 h respectively,the inhibition rate of different times showed no significant difference( P 0.05).The migratory ability of cells SKOV-3 was increased when IL-8( 100 ng / L) was added in.DDP( 100 μg/ml) inhibited IL-8 induced SKOV-3 cell migration,and as the concentration increased the number of cell migration was reduced from 241.67 to 155.99( P 0.05).Cells dealed with DDP,which NF-κB protein expression level were reduced significantly( 64.04 ± 4.6).Conclusion IL-8 can promote the migration of human ovarian cancer cells SKOV-3.DDP can inhibit the migration,and this process probably depends on the activating of the NFκB pathway.