The Effect of Inhibiting Hyperplasia of Eicosapentaenoic Acid on A-549 Human Lung Cancer Cell Line

Objective To observe the effects of anti-proliferation and inducing apoptosis on A-549 cell line induced by eicosapentaenoic acid(EPA) and its synergistic effect with cisplatin(DDP) in vitro.Methods A-549 cell line was cultured in vitro.MTT assay was used to determine the growth inhibitory effect of EPA on A-549 cells.Morphological changes of the apoptosis of A-549 cells were observed by using inverted microscope.TUNEL was used to detect cell cycle distribution and apoptotic rate of A-549 cells.Results The results of experiments showed that when A-549 cells were exposed to EPA for 24h,48h,72h and 96h respectively at final concentration of(60-120) μg/mL,the growth of cells was inhibited and showed in dose and time dependent manner.EPA combined with DDP showed significant synergistic anti-tumor effect on A-549 cells.When the concentration of EPA altered from 60μg/mL to 120μg/mL,the apoptotic index(AI) of A-549 cells elevated accordingly by the method of terminal-deoxynucleotidyl transferase mediated dUTP nick end labeling(TUNEL),the apoptotic indexes were(81.52±1.96)%,(83.74±2.21)%,(85.30±2.38)%,(86.26±2.44)% respectively and showed statistical significance compared between different concentration(P0.05).Conclusion EPA inhibits growth of A-549 cell line in vitro in dose and time dependent manner;EPA combined with DDP shows significant synergistic anti-tumor effect on A-549 cell line.