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2012•Chinese Journal of Health Laboratory TechnologyRequires access

Determination of five ginsenosides in health products by UPLC

Yan Gao

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Abstract

Objective:To develop a method for the determination of five ginsenosides(Rg1,Re,Rb1,Rc,Rd) in ginseng health products by UPLC.Methods: Samples were extracted by methanol,purified through macroporous adsorbents,and then the separation was performed on ACQUITY UPLC BEH C18 column(2.1×50 mm,1.7 μm)set at 30℃ for gradient elution,using the solution of acetonitrile and water as mobile phase at the flow rate of 0.50 ml/min and detected at 203 nm.Results: Five ginsenosides were well separated within 20 min.The calibration curves of ginsenosides showed good linearity in 39.6 μg/ml～500.0 μg/ml with correlation coefficients of 0.9995～0.9997.The detection limits of ginsenosides were 0.8 ng～ 2.5 ng.The recovery ranges were 78.6%～94.4%,and the RSDs were 2.4%～6.4%.Conclusion: The method is simple,rapid,sensitive and accurate,and suitable for the detection of five ginsenosides in health products.

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Objective:To develop a method for the determination of five ginsenosides(Rg1,Re,Rb1,Rc,Rd) in ginseng health products by UPLC.Methods: Samples were extracted by methanol,purified through macroporous adsorbents,and then the separation was performed on ACQUITY UPLC BEH C18 column(2.1×50 mm,1.7 μm)set at 30℃ for gradient elution,using the solution of acetonitrile and water as mobile phase at the flow rate of 0.50 ml/min and detected at 203 nm.Results: Five ginsenosides were well separated within 20 min.The calibration curves of ginsenosides showed good linearity in 39.6 μg/ml～500.0 μg/ml with correlation coefficients of 0.9995～0.9997.The detection limits of ginsenosides were 0.8 ng～ 2.5 ng.The recovery ranges were 78.6%～94.4%,and the RSDs were 2.4%～6.4%.Conclusion: The method is simple,rapid,sensitive and accurate,and suitable for the detection of five ginsenosides in health products.

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Available abstract

Objective:To develop a method for the determination of five ginsenosides(Rg1,Re,Rb1,Rc,Rd) in ginseng health products by UPLC.Methods: Samples were extracted by methanol,purified through macroporous adsorbents,and then the separation was performed on ACQUITY UPLC BEH C18 column(2.1×50 mm,1.7 μm)set at 30℃ for gradient elution,using the solution of acetonitrile and water as mobile phase at the flow rate of 0.50 ml/min and detected at 203 nm.Results: Five ginsenosides were well separated within 20 min.The calibration curves of ginsenosides showed good linearity in 39.6 μg/ml～500.0 μg/ml with correlation coefficients of 0.9995～0.9997.The detection limits of ginsenosides were 0.8 ng～ 2.5 ng.The recovery ranges were 78.6%～94.4%,and the RSDs were 2.4%～6.4%.Conclusion: The method is simple,rapid,sensitive and accurate,and suitable for the detection of five ginsenosides in health products.

Key concepts: Chromatography, Chemistry, Calibration curve, High-performance liquid chromatography, Gradient elution, Ginseng, Detection limit, Ginsenoside Rg1

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