Establishment and evaluation of HPLC method for determination of concentrations of ginsenosides in Life-Source Ginsenosides Complex

Objective To establish an high performance liquid chromatography(HPLC)method for the determination of concentrations of five active ginsenosides(Rg1,Re,Rb1,Rb2,and Rd)in Life-Source Ginsenosides Complex,and to provide basis for the quality control of industrial production.Methods HPLC method was used to determine the concentrations of five ginsenosides in Life-Source Ginsenosides Complex.The developed conditions of HPLC were as follows:Alltima C18(250.0mm ×4.6mm,5.0μm)column;A gradient elution was applied by mixing mobile phase A and B,which consisted of acetonitrile and 0.1% phosphoric acid, respectively;the flow rate was 1.0mL·min-1,and the detector wave length was 203 nm and the column temperature was 40℃.Results The calibration curve showed good linearity,and the values of correlation coefficient were higher than 0.999 1for all the analytes,and the average recovery rates were ranged from 99.5%to102.6%.The relative standard deviation(RSD)detected by accuracy,stability and repetability tests of five ginsenosides were 0.35%-0.76%,0.74%-1.44%,and 0.78%-1.47%,respectively.Conclusion The detection of ginsenosides in Life-Soure Ginsenosides Complex with HPLC method is accurate,easy and reproducible,which can be applied to quality control of the Life-Source Ginsenosides Complex.