Simultaneous determination of the content of 6 components in Fufang Huangliansu tablets by HPLC wavelength switching technology

Objective: To develop an HPLC method for simultaneously determining 6 components in Fufang Huangliansu tablets. Methods: The quantitative analysis was carried out on a column of Kromasil C 18( 250 mm × 4. 6 mm,5 μm) by simultaneous HPLC wavelength switching method,using a mobile phase of acetonitrile( A)- 0. 033 mol·L- 1potassium phosphate monobasic solution( B) by a gradient eluted program( 0- 15 min,9% A; 15- 55 min,9% A→35% A) with a flow rate of 1 mL·min- 1at 35 ℃. The detection wavelength was set at 230 nm for paeoniflorin in the first 25 min,and then changed to 215 nm for evodine,evodiamine,rutaecarpine,costunolide and dehydrocostuslactone between 26 and 50 min. Results: The linear ranges of paeoniflorin,evodine,evodiamine,rutaecarpine,costunolide and dehydrocostuslactone fell within the ranges of 0. 1285- 1. 285 μg,0. 2008- 2. 008 μg, 0. 1139- 1. 139 μg,0. 1151- 1. 151 μg,0. 1186- 1. 186 μg,0. 1034- 1. 034 μg,respectively. The recoveries( n = 6) of paeoniflorin,evodine,evodiamine,rutaecarpine,costunolide and dehydrocostuslactone were 99. 4%, 98. 6%,98. 7%,98. 9%,98. 9%,and 98. 6%,respectively,and the relative standard deviations were 0. 46%, 0. 64%,0. 60%,1. 1%,0. 61%,0. 38%,respectively. Conclusion: This method is simple,accurate,reproducible and convenient for the quality control of Fufang Huangliansu tablets.