2009•Central South PharmacyRequires access

Simultaneous determination of evodiamine and rutacarpine in Fufang Muxiang Xiaobojian tablets by RP-HPLC

Xiaoxiao Lu

Abstract

Objective To establish an RP-HPLC method for simultaneous determination of content of evodiamine and rutacarpine in Fufang Muxiang Xiaobojian tablets.Methods RP-HPLC and ODS2 column(250 mm×4.6 mm,5 μm) were used with acetonitrile-water(39∶61) as the mobile phase.The flow rate was 1.0 mL·min-1.The detection wavelength was 225 nm.The column temperature was 35℃.Results The linear range of evodiamine and rutacarpine was 2.096~10.480 μg·mL-1 and 2.816~14.080 μg·mL-1.The average recovery was 98.76% and 99.54% with RSD=1.68%(n=6) and RSD=1.93%(n=6) respectively.Conclusion The method established is sensitive,accurate and can be used for the quality control of Fufang Muxiang Xiaobojian tablets.

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Available abstract

Key concepts: Evodiamine, Chromatography, Chemistry, High-performance liquid chromatography, Linear range, Content determination, Detection limit