Effect of Nitrogen Source and Carbon Nitrogen Ratio on the Mycelium Growth of Pleurotus ferulae var.tuoliensis in Submerged Cultures

【Objective】The purposes of this project aim to master nutritional metabolic characteristics, analyze and extract physiologically active substances,and provide theoretical and practical guidance for further development of P.ferulae var.tuoliensis resource protection,utilization and domestication by studying nitrogen sources and carbon nitrogen ratio of mycelium growth under submerged culture condition.【Method】Six organic nitrogen sources and two inorganic nitrogen sources were added to medium of submerged cultures and eight different carbon nitrogen ratios were designed.And then the nitrogen nutritional metabolic characteristics of P.ferulae var.tuoliensis was clarified after calculating the diameter of mycelium pellet,the density of mycelium pellet and biomass of mycelium growth.【Result】The results showed that there were significant differences existing among different nitrogen sources and concentrations,which affected the mycelium growth of Pleurotus ferulae var.tuoliensis in submerged cultures.Soybean and soybean sprouts were the best nitrogen sources,the density of mycelium pellet(V/V) were 26.67%and 24.00%,and it was 145%×and 131% than the control,respectively.Peptone and yeast were better than glycine,ammonium sulphate or potassium nitrate,and urea was the worst.2.5%soybean or soybean sprouts used as nitrogen source could help us to obtain the maximum biomass,and it could be substituted for peptone.Appropriate carbon and nitrogen ratio was 20:1.【Conclusion】The metabolic capacity of P.ferulae var.tuoliensis in the different nitrogen sources was different,and the use of organic nitrogen was generally better than inorganic nitrogen.The changes in concentration of nitrogen sources affect mycelium productive capacity,and the relatively high nitrogen concentration is needed in submerged culture.