Study on Optimization of Culture Condition of Agaricus bisporus Submerged Fermentation

[Objective]With Agaricus bisporus flask incubation,the proportion of different nitrogen sources and carbon nitrogen ratio was optimized,and the optimum conditions for the growth of Agaricus bisporus was studied,so as to provide reference for wild mushroom germplasm resources.[Method]7 kinds of organic nitrogen source,2 kinds of inorganic nitrogen sources and 8 ratio of carbon to nitrogen gradient were adopted. Ball diameter,bacteria density and amount of mycelium biomass,etc were studied to inspect the flask incubation condition and nitrogen metabolic characteristics of Agaricus bisporus. [Result]Through orthogonal design test,the optimum combination of the double spore mushroom liquid fermentation and the best combination of carbon and nitrogen source and inorganic salts were studied,resulting in a suitable medium of Agaricus bisporus liquid fermentation of glucose xylose: glucose 3%,xylose 3%,peptone 0. 3%,yellow bean sprouts 5%,KH2PO40. 1%,MgSO4·7H2O 0. 05%,(NH4)2SO40. 2%,pH 5. 5,temperature 35 ℃. The optimum nitrogen source and concentration gradient significantly or extreme significantly changed the double spore mushroom growth. Soybean and bean sprouts were good nitrogen sources of Agaricus bisporus slant medium,and pellet density reached 27. 64% and 25%,which were 162% and 142% of the control. The second was peptone and yeast extract significantly,while glycine and ammonium sulfate,potassium nitrate had no significant difference between nitrogen source,nitrogen source urea was the worst. 3. 1% yellow bean sprouts and soybeans could produce maximum double spore mushroom biomass,but organic nitrogen source could be instead of peptone and yeast extract. Carbon and nitrogen ratio suitable for mushroom growth was 35∶ 1. [Conclusion]Agaricus bisporus cultivation in nitrogen sources had difference,and the organic nitrogen source was better than the other nitrogen sources,while nitrogen source gradient significantly affected the slope of the yield.