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2011•Zhongguo shiyan fangjixue zazhiRequires access

HPLC Determination of Puerarin in Kangerfu Capsule

Aidong Wen

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Abstract

Objective:HPLC method prescribed in the main component of puerarin content.Method:HPLC to Kromasil C18(4.6 mm × 250 mm,5 μm) column for the analysis of the mobile phase was methanol-water(27:73),flow rate 1 mL·min-1,detection wavelength 250 nm,Result:puerarin regression equation Y=93 128X +92 301(r=0.999 5,n=6),the scope of puerarin 0.06-0.18 μg a good linear relationship.The average recovery was 100.53%,RSD 1.41% precision.Conclusion:The method is simple,rapid,accurate,sensitive and suitable for quality control of Kanger skin.

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What this paper is about

Objective:HPLC method prescribed in the main component of puerarin content.Method:HPLC to Kromasil C18(4.6 mm × 250 mm,5 μm) column for the analysis of the mobile phase was methanol-water(27:73),flow rate 1 mL·min-1,detection wavelength 250 nm,Result:puerarin regression equation Y=93 128X +92 301(r=0.999 5,n=6),the scope of puerarin 0.06-0.18 μg a good linear relationship.The average recovery was 100.53%,RSD 1.41% precision.Conclusion:The method is simple,rapid,accurate,sensitive and suitable for quality control of Kanger skin.

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Available abstract

Objective:HPLC method prescribed in the main component of puerarin content.Method:HPLC to Kromasil C18(4.6 mm × 250 mm,5 μm) column for the analysis of the mobile phase was methanol-water(27:73),flow rate 1 mL·min-1,detection wavelength 250 nm,Result:puerarin regression equation Y=93 128X +92 301(r=0.999 5,n=6),the scope of puerarin 0.06-0.18 μg a good linear relationship.The average recovery was 100.53%,RSD 1.41% precision.Conclusion:The method is simple,rapid,accurate,sensitive and suitable for quality control of Kanger skin.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Chemistry, Capsule, Medicine, Biology, Alternative medicine

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