ScholarLens Back to search
2003•Yaoxue jinzhanRequires access

Analysis of Puerarin in Geqitongmaikeli by HPLC

Xia Zhang

Open publisher page 0 citations

Abstract

AIM To establish a HPLC mathod for the determination of Puerarin in Geqitongmaikeli.[METHODS] The column: C 18(250 mm×4.6 mm,5.0 μm ). The mobile phase: methanol-water-phosphoric acid(25∶75∶1). The content of Puerarin was detected at 250 nm. [RESULTS] The linear range of the determination by HPLC is 16～56 μg/ml, the correlation coefficient is 0.999 9, the sample solution is stable within 24 h and the precision is good (RSD 0.15%).The recovery results are 100.17%. [CONCLUSION] The method is easy to operate and accurate.

About this research paper

What this paper is about

AIM To establish a HPLC mathod for the determination of Puerarin in Geqitongmaikeli.[METHODS] The column: C 18(250 mm×4.6 mm,5.0 μm ). The mobile phase: methanol-water-phosphoric acid(25∶75∶1). The content of Puerarin was detected at 250 nm. [RESULTS] The linear range of the determination by HPLC is 16～56 μg/ml, the correlation coefficient is 0.999 9, the sample solution is stable within 24 h and the precision is good (RSD 0.15%).The recovery results are 100.17%. [CONCLUSION] The method is easy to operate and accurate.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

AIM To establish a HPLC mathod for the determination of Puerarin in Geqitongmaikeli.[METHODS] The column: C 18(250 mm×4.6 mm,5.0 μm ). The mobile phase: methanol-water-phosphoric acid(25∶75∶1). The content of Puerarin was detected at 250 nm. [RESULTS] The linear range of the determination by HPLC is 16～56 μg/ml, the correlation coefficient is 0.999 9, the sample solution is stable within 24 h and the precision is good (RSD 0.15%).The recovery results are 100.17%. [CONCLUSION] The method is easy to operate and accurate.

Key concepts: Puerarin, Chromatography, High-performance liquid chromatography, Phosphoric acid, Correlation coefficient, Linear correlation, Chemistry, Linear relationship

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Analysis of Puerarin in Geqitongmaikeli by HPLC — Research Paper | ScholarLens