Study on Genetic Toxicity of Propolic

Objective To investigate the genetic toxicity of propolic so as to assess its safety.Methods Ames Test was conducted using histidine requiring Salmonella typhimurium indicator strains TA97 (a),TA98,TA100 and TA102.The arginine esterase was tested with the plate incorporation assay with and without metabolic activation system S9 mixture at five concentrations,namely,0.005,0.025,0.250,1.000,5.000/plate.Cell micronucleus test of marrow was used.The micronucleus polychromatic erythrocytes of marrow were observed and measured.Sperm shape abnormality test was performed to assess the deform numbers of sperm.Resultes In Ames test,the mutagenicity of propolic group was no more than 2 times that of the control group.Micronucle rate and deform numbers of sperm in different groups of propolic were all negative(compared with negative control P0.05,compared with positive control P0.05).Conclusion The propolic is not mutagenic to tested strains,somatic cell and male germ cells in mice.