Study on genetic toxicity of Pollen Pini

Objective To investigate the genetic toxicity of pollen pini in order to assess its safety.Methods Ames Test was conducted by using histidine requiring Salmonella typhimurium indicator strains TA97(a),TA98,TA100 and TA102.The arginine esterase was detected by plate incorporation assay with and without metabolic activation system S 9 mixture at five concentrations,namely,0.008,0.040,0.200,1.000,5.000/plate.Cell micronucleus test of marrow was used.The micronucleus polychromatic erythrocytes of marrow were observed and measured.The sperm shape abnormality test was performed to assess the deform numbers of sperm.Results In Ames test,the mutagenicity of pollen pini group was less than 2 times,as compared with that of control group.There were no significant difference in micronucleus rate and deform numbers of sperm between pollen pini groups and negative control group(P0.05),but there was a significant difference between pollen pini groups and positive control group(P0.05).Conclusion The pollen pini is not mutagenic to tested trains,somatic cell and male germ cells in mice.