Extraction and properties on scale collagen

This experiment with fish processing scales for research object of scraps,Adopt pepsin respectively in acid(hydrochloric acid,acetic acid,citric acid) conditions zymolysing get collagen.Explore acid concentration,enzymatic hydrolysis time,enzymatic hydrolysis temperature and the influence of material liquid extraction ratio,And to get zymolysing collagen freeze-drying purification for further study of nature.Test results show that:Extraction rate from high to low in turn is citric acidacetic acid hydrochloric acid,The factors which effect of acetic acid extract order size is acetic acid concentrationextraction temperature material liquidextraction time.The scheme for best test is acetic acid concentration 0.6 mol/L,Extraction temperature is 36 ℃,Extraction time is 6 h,Than liquid materials is 1:30,Now hydroxyproline content is 2.7522 mg/mL,Extraction rate reached 73.32%.Nature research results indicate:Collagen solubility is very good,Emulsification is hydrochloric acid extraction is highest,59.6%,Emulsion stability are good,and suck oily,foaming ability is the best acetic acid extraction,Achieved respectively is 3 mL/g,90%.