Extraction of Collagen from Fish Scale by Microwave Method and Study on Its Properties

Collagen was extracted from byproduct of fish,fish scale,by microwave extraction method.The effects of acetic acid concentration in extract solvent,microwave power,microwave treatment time and material to solvent ratio on the extraction yield of collagen from fish scale were analyzed by single factor tests and orthogonal experiment.The results showed that the optimized extraction technology was using 0.6 mol/L acetic acid as extract solvent with material to solvent ratio at mscale∶ Vacetic acid=1∶25(g/mL),extract for 5 min under 400 W of microwave power.The content of hydroxyproline in extractant solution was 186.358 mg/g under the extraction technology;while the extraction yield of collagen was 41.37%.According to property tests,the water absorption,solubility,emulsibility,emulsion stability,oil absorption and foamability of the extracted collagen was 0.466 g/g,100%,51.67%,91%,2.8 mL/g and 84%,respectively.The comprehensive properties of the collagen were good.