Effect of jiejufushenning on the secretion and expressions of platelet-derived growth factor-B in rat glomerular mesangial cells cultivated under high glucose

Objective To investigate the effect of jiejufushenning(JJFSN) on the secretion,expressions of platelet-derived growth factor-B(PDGF-B) in rat glomerular mesangial cells(GMCs) cultivated under high glucose.Methods The two drug-contained serum of model rats were prepared,then GMCs were cultured with high concentration glucose(HG) and HG plus irbesartan,different dose of JJFSN for different duration(24,48,72 h),and set up normal control simultaneously.The content of platelet-derived growth factor-B(PDGF-B) in the supernatant of cultured GMCs were detected by ELISA,and the expressions of PDGF-B were detected by RT-PCR,Western Blot,respectively.Results Compared with normal control group,the content of secretion of PDGF-B in the supernatant of cultured GMCs was higher in HG(P0.001),and the expression of PDGF-B was up-regulated in HG group(P0.001).Compared with HG group,HG plus irbesartan,and high,middle,low dose of JJFSN significantly decreased PDGF-B secretion in the supernatant of cultured GMCs(P0.005),and decreased the expressions of PDGF-B,JJFSN groups were more significant(P0.05),a certain dose-dependent and time-dependent was shown.Conclusions GMCs can increase secretion and expressions of PDGF-B induced by high glucose,JJFSN can reduce the secretion of PDGF-B,and down-regulate the expressions of PDGF-B,then delay the progression of glomerular sclerosis of diabetic nephropathy.