Effect of enalapril on the production of transforming growth factor-β1,laminin and connective tissue growth factor in rat glomerular mesangial cells cultivated under high glucose

Objective To investigate the effect of enalapril on the production of transforming growth factor-β1(TGF-β1),laminin(LN) and connective tissue growth factor(CTGF) in rat glomerular mesangial cells(GMCs) cultivated under high glucose. Methods GMCs were cultured with normal concentration glucose(NG,5.5 mmol/L),high concentration glucose(HG,20 mmol/L),and HG plus different concentration of enalapril for different duration(24 h,48 h,72 h),and blank control was set up simultaneously. The contents of TGF-β1,LN in the supernatant of cultured. GMCs were detected by RIA,ELISA,respectively,and the expression of CTGF was detected by RT-PCR. Results Compared with those in blank control group,the contents of TGF-β1,LN were higher than those in NG and HG groups (P0.001),and the expression of CTGF was up-regulated in NG and HG groups (P0.001),HG group was more significant(P0.001). Compared with those in HG group,HG plus high,middle,low concentration of enalapril groups had significantly lower TGF-β1,LN in the supernatant of cultured GMCs(P0.005),and decreased the expression of CTGF. Conclusions GMCs can increase the secretion of TGF-β1,synthesis of LN,expression of CTGF induced by high glucose. Enalapril can reduce accumulation of extracelluar matrix(ECM),and down-regulate the expression of CTGF,then delay the progression of glomerular sclerosis of diabetic nephropathy.