Effects of Tangshen Particle on the secretion and expression of midkine in rat glomeruli mesangial cells cultivated under high glucose

Objective:To investigate the effect of Tangshen Particle on the secretion,expression of midkine(MK) in rat Glomeruli Mesangial Cells(GMCs) cultivated under high glucose.Methods:Two drug-contained serum of model rats were prepared,then GMCs were cultured with high concentration glucose(HG) and HG plus Benazepril,different doses of Tangshen Particle for different duration(24,48,72h),and set up normal control simultaneously.The content of midkine(MK) in the supernatant of cultured GMCs were detected by ELISA,and the expression of MK was detected by RT-PCR,Western Blot,respectively.Results:Compared with the normal control group,the content of secretion of MK in the supernatant of cultured GMCs was higher in HG(P0.001).Compared with HG group,Benazepril group and Tangshen Particle group decreased the expression of MK,Tangshen Particle groups were more significant(P0.05),a certain dose-dependent and time-dependent was shown.Conclusion:GMCs can increase the secretion and expression of MK induced by high glucose,Tangshen Particle can reduce the secretion of MK,and down-regulate the expression of MK,then delay the progress of glomerular sclerosis of diabetic nephropathy.