Study on Bulb Scale Culture in vitro of Lilium concolor var.buschianum

The bulb scales of Lilium concolor var.buschianum were used as explants to study the effects of different hormone combination on adventitious buds induction,proliferation and rooting.And the effect of different media on cutting survival of tube plantlets was investigated.The results showed that the appropriate medium for the adventitious buds induction was MS+BA 0.5 mg/L+NAA 0.5 mg/L with the induction rate of 80.0%.The suitable subculture medium was MS+BA 1.0 mg/L+NAA 0.1 mg/L with the multiplication coefficient of 2.83.The appropriate medium for rooting was MS+IBA 0.5 mg/L with the rooting rate of 100.0%.The proper medium for cutting of tube plantlets was perlite with the survival rate of 96.7%.An effective system of regeneration and multiplication of bulb scales of Lilium concolor var.buschianum was established.