Study on bulb scale culture in vitro of Lilium cernuum Komar.

The bulb scales of Lilium cernuum Komar.were used as explants for in vitro culture.The results showed that the best disinfection method of bulb scale explants of Lilium cernuum was 70% ethanol for 30 s,followed by 0.1% HgCl2 12 min with the contamination rate of 13.3% and the survival rate of 92.6%.The capacity of adventitious buds induction of scales in different parts was the lowerthe middlethe upper,with the induction rate of 75.0%,68.3% and 38.3% respectively and the number of adventitious buds per explant of 2.7,2.3 and 1.5 respectively.The induction rate and the number of adventitious buds per explant from the lower scales were the highest and significantly higher than from the upper scales.The appropriate media for the induction of adventitious buds were MS+BA 0.5 mg·L-1+NAA 0.5 mg·L-1 and MS+BA 1.0 mg·L-1+NAA 1.0 mg·L-1 with the induction rates of 67.5% and 72.5% respectively,and the number of adventitious buds per explant of 2.4 and 2.2 respectively.The appropriate medium for multiplication was MS+BA 0.5 mg·L-1+NAA 1.0 mg·L-1 with the multiplication coefficient of 2.9.The appropriate medium for rooting was 1/2MS+IBA 0.5 mg·L-1 with the rooting rate of 99.4% and the number of roots per plantlets of 9.6.The proper substrate for cutting was sand with the survival rate of 94.0%.