Study of Tissue Culture and Establishment of Asexual System of Lilium concolor var.buschianum
Jiang Chang-yang
Abstract
Jiang Chang-yang
Abstract
A study was conducted on the induction and differentiation of callus,bud differentiation,rooting,transplanting,cuttage of test tube seedling for Lilium concolor var.buschianum.The results indicated that the ideal medium for induction of callus from young stem was MS+BA 0.4 mg/L+2,4-D 1.6 mg/Land MS+ BA 0.4 mg/L+NAA 0.2 mg/L was ideal medium for differentiation of callus and adventitious bud.The medium of 1/3MS+IAA 0.4 mg/L was suitable for root growth.The test tube seedling transplanted has the character with exuberant growth and the developed root system.
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A study was conducted on the induction and differentiation of callus,bud differentiation,rooting,transplanting,cuttage of test tube seedling for Lilium concolor var.buschianum.The results indicated that the ideal medium for induction of callus from young stem was MS+BA 0.4 mg/L+2,4-D 1.6 mg/Land MS+ BA 0.4 mg/L+NAA 0.2 mg/L was ideal medium for differentiation of callus and adventitious bud.The medium of 1/3MS+IAA 0.4 mg/L was suitable for root growth.The test tube seedling transplanted has the character with exuberant growth and the developed root system.
Key concepts: Callus, Seedling, Transplanting, Biology, Botany, Lilium, Murashige and Skoog medium, Tissue culture