The protective effect of glutathione(GSH) on the pancreas and liver injury in rats with early severe acute pancreatitis

Objective To study the protective effect of glutathione(GSH) on the pancreas and liver injury in rats with early severe acute pancreatitis(SAP).Methods 54 male Wistar rats were randomly divided into three groups(n=18): group A,18 male Wistar rats for normal control group,just flip the pancreas after laparotomy,the abdomen was closed;group B,18 male Wistar rats for the SAP group,4.5% sodium taurocholate(1 mL/kg) was retrograded into the pancreatic duct at the rate of 0.1 mL/min;group C,18 male Wistar rats,N-acetylcysteine(NAC) treatment group,half hour before surgery,NAC(200 mg/kg) was given by intraperitoneal injection,followed by the establishment of a rat model of SAP.6 hours after surgery,rats were anesthetized to obtain fresh pancreas and liver tissue,to take part for GSH detection and one for pathological observation and electron microscopy.Results GSH levels in pancreatic tissue: the control group NAC treated group SAP group,there were significantly different(P0.05).Pancreatic histopathological examination and scoring: SAP group and NAC treated group rats occurred acute necrotizing pancreatitis lesions,but the pancreatic necrosis in NAC treatment group lessened than that in the SAP group,there were significantly different between SAP group and NAC treatment group(P=0.0368,P0.05).Electron microscopy after six hours: N-acetylcysteine could improve the zymogen granules within the pancreatic acinar cell operation.Liver ultrastructure: endoplasmic reticulumed and mitochondrial swellinged in SAP group;partial visible endoplasmic reticulumed in the NAC treatment group.Conclusion With the GSH consumption,the incidence of early(6 hours) can occur the damage of pancreas and liver,supplement of N-acetylcysteine can reduce the damage of the pancreas and liver.