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2013•Anhui nongye kexueRequires access

Extracting Atractylodes macroephala DNA by Using CTAB and SDS Method

Heng Huang

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Abstract

[Objective] To obtain high-quality DNA from Atractylodes macroephala.[Method] Selecting fresh leaf of A.macroephala,broken cell wall,CTAB and SDS method were adopted to conduct DNA isolation and purification.[Result] The results showed that OD value by using improved CTAB method is 1.8-1.9,which is higher than OD value 1.5-1.6 by using improved SDS method.[Conclusion] DNA concentration and purification by using CTAB method is higher,which can be used as templet in subsequent molecular markers.

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What this paper is about

[Objective] To obtain high-quality DNA from Atractylodes macroephala.[Method] Selecting fresh leaf of A.macroephala,broken cell wall,CTAB and SDS method were adopted to conduct DNA isolation and purification.[Result] The results showed that OD value by using improved CTAB method is 1.8-1.9,which is higher than OD value 1.5-1.6 by using improved SDS method.[Conclusion] DNA concentration and purification by using CTAB method is higher,which can be used as templet in subsequent molecular markers.

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Available abstract

[Objective] To obtain high-quality DNA from Atractylodes macroephala.[Method] Selecting fresh leaf of A.macroephala,broken cell wall,CTAB and SDS method were adopted to conduct DNA isolation and purification.[Result] The results showed that OD value by using improved CTAB method is 1.8-1.9,which is higher than OD value 1.5-1.6 by using improved SDS method.[Conclusion] DNA concentration and purification by using CTAB method is higher,which can be used as templet in subsequent molecular markers.

Key concepts: Atractylodes, DNA, Chromatography, DNA extraction, Chemistry, Biochemistry, Medicine, Gene

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