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2010•Xinjiang nongye kexueRequires access

Comparison of methods of genomics DNA extraction from Caperberries.

Shi QinHua, Dai PeiHong, Xu Lei, Shubing Shi

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Abstract

[Objective]To get the best method of DNA extraction from Caperberries(Capparis spinosa L.).[Method] CTAB method,SDS-CTAB method,SDS method and Urea method were used to extract genomic DNA from the leaves of 3 species of Caperberries.[Result]The extracted DNA samples were tested using A260/A280,agarose gel electrophoresis.The difference in purity,integrity,yield,time consumption of the obtained DNA were compared with one another.[Conclusion]CTAB method was the best one of the four methods.

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What this paper is about

[Objective]To get the best method of DNA extraction from Caperberries(Capparis spinosa L.).[Method] CTAB method,SDS-CTAB method,SDS method and Urea method were used to extract genomic DNA from the leaves of 3 species of Caperberries.[Result]The extracted DNA samples were tested using A260/A280,agarose gel electrophoresis.The difference in purity,integrity,yield,time consumption of the obtained DNA were compared with one another.[Conclusion]CTAB method was the best one of the four methods.

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Available abstract

[Objective]To get the best method of DNA extraction from Caperberries(Capparis spinosa L.).[Method] CTAB method,SDS-CTAB method,SDS method and Urea method were used to extract genomic DNA from the leaves of 3 species of Caperberries.[Result]The extracted DNA samples were tested using A260/A280,agarose gel electrophoresis.The difference in purity,integrity,yield,time consumption of the obtained DNA were compared with one another.[Conclusion]CTAB method was the best one of the four methods.

Key concepts: DNA extraction, genomic DNA, DNA, Agarose gel electrophoresis, Chromatography, Extraction (chemistry), Biology, Agarose

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