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2011•Anhui nongye kexueRequires access

Study on in vitro Rapid Propagation and Plant Regeneration of Leptopyrum fumarioides

Xiaoshan Duan

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Abstract

[Objective]To provide platform for the domestication and transgene research of Leptopyrum fumarioides.[Method]The effects of explants type,plant growth regulator type and concentration ratio on the callus induction and adventitious buds differentiation of this species were studied.[Results]With the leaves as the explants,the optimized callus induction medium was MS + 6-BA 0.5 mg/L + IBA 3.0 mg/L,and the optimized medium for the differentiation of adventitious buds was MS + 6-BA 0.5 mg/L + IBA 1.0 mg/L.

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What this paper is about

[Objective]To provide platform for the domestication and transgene research of Leptopyrum fumarioides.[Method]The effects of explants type,plant growth regulator type and concentration ratio on the callus induction and adventitious buds differentiation of this species were studied.[Results]With the leaves as the explants,the optimized callus induction medium was MS + 6-BA 0.5 mg/L + IBA 3.0 mg/L,and the optimized medium for the differentiation of adventitious buds was MS + 6-BA 0.5 mg/L + IBA 1.0 mg/L.

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Available abstract

[Objective]To provide platform for the domestication and transgene research of Leptopyrum fumarioides.[Method]The effects of explants type,plant growth regulator type and concentration ratio on the callus induction and adventitious buds differentiation of this species were studied.[Results]With the leaves as the explants,the optimized callus induction medium was MS + 6-BA 0.5 mg/L + IBA 3.0 mg/L,and the optimized medium for the differentiation of adventitious buds was MS + 6-BA 0.5 mg/L + IBA 1.0 mg/L.

Key concepts: Explant culture, Callus, Regeneration (biology), In vitro, Botany, Plant growth, Murashige and Skoog medium, Biology

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