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[Plant regeneration from callus of Changiums myrnioides].

Xiuquan Xu, Chunyan Yan, Rongmin Yu

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Abstract

OBJECTIVE: To set up the callus culture system of Changiums myrnioides and the plantlet regeneration system by the proliferation and differentiation of callus. METHOD: The segment of stems used as the explants were cultured on dedifferentiation and differentiation medium supplied with different auxin and cytokinin. RESULT: MS medium with NAA 0.5 - 1.0 mg/L + KT 0.1 mg/L was best combination for the induction of callus, MS medium with 6 - BA 1 - 2 mg/L + GA 1 - 2 mg/L was suitable for the differentiation of adventitious buds, and MS medium with IBA 0.5 - 1 mg/L was suitable for the induction of root. CONCLUSION: Callus culture may be used as a useful method to get regenerated plantlet of Changiums myrnioides under certain condition.

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OBJECTIVE: To set up the callus culture system of Changiums myrnioides and the plantlet regeneration system by the proliferation and differentiation of callus. METHOD: The segment of stems used as the explants were cultured on dedifferentiation and differentiation medium supplied with different auxin and cytokinin. RESULT: MS medium with NAA 0.5 - 1.0 mg/L + KT 0.1 mg/L was best combination for the induction of callus, MS medium with 6 - BA 1 - 2 mg/L + GA 1 - 2 mg/L was suitable for the differentiation of adventitious buds, and MS medium with IBA 0.5 - 1 mg/L was suitable for the induction of root. CONCLUSION: Callus culture may be used as a useful method to get regenerated plantlet of Changiums myrnioides under certain condition.

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Available abstract

OBJECTIVE: To set up the callus culture system of Changiums myrnioides and the plantlet regeneration system by the proliferation and differentiation of callus. METHOD: The segment of stems used as the explants were cultured on dedifferentiation and differentiation medium supplied with different auxin and cytokinin. RESULT: MS medium with NAA 0.5 - 1.0 mg/L + KT 0.1 mg/L was best combination for the induction of callus, MS medium with 6 - BA 1 - 2 mg/L + GA 1 - 2 mg/L was suitable for the differentiation of adventitious buds, and MS medium with IBA 0.5 - 1 mg/L was suitable for the induction of root. CONCLUSION: Callus culture may be used as a useful method to get regenerated plantlet of Changiums myrnioides under certain condition.

Key concepts: Callus, Plantlet, Explant culture, Regeneration (biology), Botany, Auxin, Cytokinin, Murashige and Skoog medium

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