Optimization of Purification Technology for Total Flavonoids in Bidens bipinnata by Macroporous Adsorption Resin

Objective To optimize purification technology of total flavonoids in Bidens bipinnata by macroporous resin. Method With adsorption rate and desorption rate of total flavonoids as indexes, adsorption property of six different kinds of macroporous resins for total flavonoids in B. bipinnata was compared by static adsorption and desorption experiments, in order to select optimum macroporous resin. Single factor test was used to investigate adsorption and elution conditions of macroporous resin. Result HPD400 macroporous resin presented the best effect of purification. Its optimum purification parameters were as followings: the sample concentration 0.5 g/L,pH 4,sample flow rate 2 BV/h,eluted with 9 BV 60% ethanol at 3 BV/h. After purification by macroporous resin, purity of total flavonoids in ethanol extract of B. bipinnata would be increased from 24.47% to 62.33%. Conclusion HPD400 macroporous resin was among the most suitable one for purification of total flavonoids in B. bipinnata. Optimized purification technology was stable and feasible.