ScholarLens Back to search
2005•Chinese Journal of Veterinary DrugRequires access

Determination of benzimidazoles residues in beef liver by HPLC

Zhangqi Shen

Open publisher page 0 citations

Abstract

A method of high performance liquid chromatographg (HPLC)was developed to determine benzi- midazoles residues in beef liver. Benzimidazoles were extracted from tissues by ethyl acetate, purified by hexane and C 18 cartridge, separated by HPLC on an ODS column and measured by ultraviolet detector with the mobile phase of methanol-phosphate buffer. The average recovery was 78.8% with coefficient of variation of 2.18%. The detection limit was 8 μg/kg and the quantitation limit was 25 μg/kg.

About this research paper

What this paper is about

A method of high performance liquid chromatographg (HPLC)was developed to determine benzi- midazoles residues in beef liver. Benzimidazoles were extracted from tissues by ethyl acetate, purified by hexane and C 18 cartridge, separated by HPLC on an ODS column and measured by ultraviolet detector with the mobile phase of methanol-phosphate buffer. The average recovery was 78.8% with coefficient of variation of 2.18%. The detection limit was 8 μg/kg and the quantitation limit was 25 μg/kg.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

A method of high performance liquid chromatographg (HPLC)was developed to determine benzi- midazoles residues in beef liver. Benzimidazoles were extracted from tissues by ethyl acetate, purified by hexane and C 18 cartridge, separated by HPLC on an ODS column and measured by ultraviolet detector with the mobile phase of methanol-phosphate buffer. The average recovery was 78.8% with coefficient of variation of 2.18%. The detection limit was 8 μg/kg and the quantitation limit was 25 μg/kg.

Key concepts: Chromatography, High-performance liquid chromatography, Chemistry, Cartridge, Hexane, Coefficient of variation, Detection limit, Ethyl acetate

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Determination of benzimidazoles residues in beef liver by HPLC — Research Paper | ScholarLens