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2002•Chinese Journal of Veterinary DrugRequires access

Determination of Chloramphenicol Residues in Milk by HPLC

Yue Xiu-ying

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Abstract

It was described the method for isolating and determinating chloramphenicol residues in milk. Chloram-phenicol in milk samples was extracted by ethyl acetate, and the extracts were purified with a mixture of chloro-form-hexane(1+1, V/V). Chloramphenicol was quantified by high performmmance liquid chromatography (HPLC)on silica coated column type C18 with UV detection at 278 nm, and with the mobile phase of acetonitrile-0.005mol/L ammonium hydrogen phosphate(18+82, V/V). The results showed that the average recovery was 94.76%,and average coefficient of variation was less than 12.0%.

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What this paper is about

It was described the method for isolating and determinating chloramphenicol residues in milk. Chloram-phenicol in milk samples was extracted by ethyl acetate, and the extracts were purified with a mixture of chloro-form-hexane(1+1, V/V). Chloramphenicol was quantified by high performmmance liquid chromatography (HPLC)on silica coated column type C18 with UV detection at 278 nm, and with the mobile phase of acetonitrile-0.005mol/L ammonium hydrogen phosphate(18+82, V/V). The results showed that the average recovery was 94.76%,and average coefficient of variation was less than 12.0%.

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Available abstract

It was described the method for isolating and determinating chloramphenicol residues in milk. Chloram-phenicol in milk samples was extracted by ethyl acetate, and the extracts were purified with a mixture of chloro-form-hexane(1+1, V/V). Chloramphenicol was quantified by high performmmance liquid chromatography (HPLC)on silica coated column type C18 with UV detection at 278 nm, and with the mobile phase of acetonitrile-0.005mol/L ammonium hydrogen phosphate(18+82, V/V). The results showed that the average recovery was 94.76%,and average coefficient of variation was less than 12.0%.

Key concepts: Chloramphenicol, Chromatography, High-performance liquid chromatography, Chemistry, Ammonium acetate, Coefficient of variation, Hexane, Acetonitrile

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