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2006•Unpublished venueRequires access

Analysis of asarone and impurities in asarone injection by HPLC

Ting Zhang

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Abstract

Objective To establish an HPLC method for the determination of asarone and impurities in asarone injection.Methods The analysis was performed on a Kromasil-C_18 column using methanol-water(80∶20)as mobile phase and the detection wavelength was 313 nm.Results Asarone and impurities could be separated well under the condition.Linear calibration curve was obtained in the range of 2～80 mg·L~-1 with an average recovery of 100.2%(RSD= 0.37%).Conclusion The method can be used for the quality control of asarone injection.

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What this paper is about

Objective To establish an HPLC method for the determination of asarone and impurities in asarone injection.Methods The analysis was performed on a Kromasil-C_18 column using methanol-water(80∶20)as mobile phase and the detection wavelength was 313 nm.Results Asarone and impurities could be separated well under the condition.Linear calibration curve was obtained in the range of 2～80 mg·L~-1 with an average recovery of 100.2%(RSD= 0.37%).Conclusion The method can be used for the quality control of asarone injection.

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Available abstract

Objective To establish an HPLC method for the determination of asarone and impurities in asarone injection.Methods The analysis was performed on a Kromasil-C_18 column using methanol-water(80∶20)as mobile phase and the detection wavelength was 313 nm.Results Asarone and impurities could be separated well under the condition.Linear calibration curve was obtained in the range of 2～80 mg·L~-1 with an average recovery of 100.2%(RSD= 0.37%).Conclusion The method can be used for the quality control of asarone injection.

Key concepts: Impurity, Calibration curve, Chromatography, High-performance liquid chromatography, Chemistry, Methanol, Detection limit, Organic chemistry

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