Amniotic cells culture and its application in the prenatal diagnosis

Objective:To explore an amniotic cell culture technology with higher harvests and verify its validity in prenatal diagnosis.Methods: The amniotic fluid samples were obtained in 238 pregnant women with gestational weeks of 16 to 30 and indications for prenatal diagnosis by aseptic amniocentesis and cultured for determination of the chromosome karyotype.In real performance,moderate improvement was done to the sample collection,cell inoculation and harvesting as well as aspects involved in the procedures.Results: Amniotic cells were finally cultured in 231 of 238 cases(97.1%)whose abnormalities were identified in 8(3.5%)in which 6 were abnormal number of chromosomes,1mosaic and another 1,chromosomal balance translocation.Conclusion: The modified amniotic cell culturing technique for kargotyping is effective in prenatal diagnosis of fetal chromosomal disease for its safety and reliability.