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2011•Zhonghua zhongyiyao xuekanRequires access

Simultaneous Determination of Rutin,Hyperoside,Quercetin in Wild Fructus Crataegi by Multiple Wavelength RP-HPLC

Bing Wang

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Abstract

Objective:To establish a method for simultaneous determination of rutin,hyperoside,quercetin in Fructus Crataegi.Methods:A multiple wavelength HPLC method was developed.The analysis was performed on an Agilent EclipseXDB-C18 column(4.6mm×150mm,5μm).A mixture of acetonitrile(A)-0.025mol/L phosphoric acid(B)with gradient elution as the mobile phase was adopted.The gradient elution program was 0～15min(18%A～18%A),15～30min(18%A～40%A).The wavelength was monitored 363nm(0～18min),371nm(18～30min).The flow rate was 0.9mL/min.The column temperature was 28℃.Results:The linear response ranges of rutin,hyperoside and quercetin were 0.06296～0.3148μg,1.0512～5.2560μg,0.06240～0.3120μg respectively.The average recoveries were 100.59%,99.27%,100.03% with RSD of 2.00%,1.99%,1.92% respectively.Conclusion:The method is simple and reproducible for determining rutin,hyperoside,quercetin in Folium Crataegi.

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What this paper is about

Objective:To establish a method for simultaneous determination of rutin,hyperoside,quercetin in Fructus Crataegi.Methods:A multiple wavelength HPLC method was developed.The analysis was performed on an Agilent EclipseXDB-C18 column(4.6mm×150mm,5μm).A mixture of acetonitrile(A)-0.025mol/L phosphoric acid(B)with gradient elution as the mobile phase was adopted.The gradient elution program was 0～15min(18%A～18%A),15～30min(18%A～40%A).The wavelength was monitored 363nm(0～18min),371nm(18～30min).The flow rate was 0.9mL/min.The column temperature was 28℃.Results:The linear response ranges of rutin,hyperoside and quercetin were 0.06296～0.3148μg,1.0512～5.2560μg,0.06240～0.3120μg respectively.The average recoveries were 100.59%,99.27%,100.03% with RSD of 2.00%,1.99%,1.92% respectively.Conclusion:The method is simple and reproducible for determining rutin,hyperoside,quercetin in Folium Crataegi.

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Available abstract

Objective:To establish a method for simultaneous determination of rutin,hyperoside,quercetin in Fructus Crataegi.Methods:A multiple wavelength HPLC method was developed.The analysis was performed on an Agilent EclipseXDB-C18 column(4.6mm×150mm,5μm).A mixture of acetonitrile(A)-0.025mol/L phosphoric acid(B)with gradient elution as the mobile phase was adopted.The gradient elution program was 0～15min(18%A～18%A),15～30min(18%A～40%A).The wavelength was monitored 363nm(0～18min),371nm(18～30min).The flow rate was 0.9mL/min.The column temperature was 28℃.Results:The linear response ranges of rutin,hyperoside and quercetin were 0.06296～0.3148μg,1.0512～5.2560μg,0.06240～0.3120μg respectively.The average recoveries were 100.59%,99.27%,100.03% with RSD of 2.00%,1.99%,1.92% respectively.Conclusion:The method is simple and reproducible for determining rutin,hyperoside,quercetin in Folium Crataegi.

Key concepts: Hyperoside, Rutin, Chromatography, Quercetin, Phosphoric acid, Gradient elution, High-performance liquid chromatography, Chemistry

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Simultaneous Determination of Rutin,Hyperoside,Quercetin in Wild Fructus Crataegi by Multiple Wavelength RP-HPLC — Research Paper | ScholarLens