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2013•Zhonghua zhongyiyao xuekanRequires access

HPLC Determination of Rutin Hyperoside Quercetin in Hawthorn Extracts

Wei Liu

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Abstract

Objective:To establish an HPLC method for determination of rutin,hyperoside and quercetin in hawthorn extract.Methods:The separation was performed on a Phenomsil-C18 ODS(250 mm×4.6 mm,5 μm) column with the gradient elution of acetonitrile-water of 0.5% phosphoric acid at the flow rate of 1.0 mL·min-1.Detection wavelength was 360 nm.The column temperature was set at 30 ℃.Results:Rutin,hyperoside and quercetin had good linearity in the ranges of 0.0900～0.900,0.1077～1.077,0.03405～0.3405 μg,repectively.The average recoveries of matters were 95.9%～101.6%,with RSDs of the recoveries were less than 2.1%.Conclusion:The developed method is helpful to control the quality of hawthorn extract.

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What this paper is about

Objective:To establish an HPLC method for determination of rutin,hyperoside and quercetin in hawthorn extract.Methods:The separation was performed on a Phenomsil-C18 ODS(250 mm×4.6 mm,5 μm) column with the gradient elution of acetonitrile-water of 0.5% phosphoric acid at the flow rate of 1.0 mL·min-1.Detection wavelength was 360 nm.The column temperature was set at 30 ℃.Results:Rutin,hyperoside and quercetin had good linearity in the ranges of 0.0900～0.900,0.1077～1.077,0.03405～0.3405 μg,repectively.The average recoveries of matters were 95.9%～101.6%,with RSDs of the recoveries were less than 2.1%.Conclusion:The developed method is helpful to control the quality of hawthorn extract.

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Available abstract

Objective:To establish an HPLC method for determination of rutin,hyperoside and quercetin in hawthorn extract.Methods:The separation was performed on a Phenomsil-C18 ODS(250 mm×4.6 mm,5 μm) column with the gradient elution of acetonitrile-water of 0.5% phosphoric acid at the flow rate of 1.0 mL·min-1.Detection wavelength was 360 nm.The column temperature was set at 30 ℃.Results:Rutin,hyperoside and quercetin had good linearity in the ranges of 0.0900～0.900,0.1077～1.077,0.03405～0.3405 μg,repectively.The average recoveries of matters were 95.9%～101.6%,with RSDs of the recoveries were less than 2.1%.Conclusion:The developed method is helpful to control the quality of hawthorn extract.

Key concepts: Hyperoside, Rutin, Chromatography, Quercetin, Phosphoric acid, Chemistry, High-performance liquid chromatography, Gradient elution

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