2008•Northern HorticultureRequires access

Rapid Propagation in Vitro of Stem-segment with Single Bud of Chrysanthemum

Xiangping Yan

Abstract

Withminimal media MS axillary innovations of Chrysanthemum recommended variety 'Wansheng' were used as explants to be cultured in vitro.The results showed that MS+BA 2.0～3.0mg/L+NAA 0.1mg/Lwas the appropriate medium to induce buds,MS+BA 0.5mg/L+NAA 0.1mg/L was the most effective medium for subculture,and 1/2 MS+IBA 0.15mg/L was ideal medium to root.Hardening seedlings in perlite,the survival rate could be 100%.The similar result was obtained after transplanting tube seedlings to nutritive soil directiy with survival rate being 95.6%.The latter method was proved easily and effectually.

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Key concepts: Transplanting, Axillary bud, Perlite, Explant culture, Subculture (biology), In vitro, Vermiculite, Murashige and Skoog medium