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2006•Chinese Journal of Tropical AgricultureRequires access

Establishment of Efficient Plant Regeneration System from Leaves of Chrysanthemum

Chen Xiong-ting

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Abstract

A plant regeneration system from leaves of chrysanthemum was developed in this study. The optimum media were obtained for callus induction, shoot regeneration and rooting. Calli were induced from leaf explants cultured on the medium MS + BA 2 mg/L + NAA 0.2 mg/L. Bud shoots were induced from the calli cultured on the medium MS + BA 3 mg/L + NAA 0.1 mg/L, and rooted on the half-strength MS medium at the presence of BA 0.6 mg/L. The rooted plantlets survived in greenhouse.

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What this paper is about

A plant regeneration system from leaves of chrysanthemum was developed in this study. The optimum media were obtained for callus induction, shoot regeneration and rooting. Calli were induced from leaf explants cultured on the medium MS + BA 2 mg/L + NAA 0.2 mg/L. Bud shoots were induced from the calli cultured on the medium MS + BA 3 mg/L + NAA 0.1 mg/L, and rooted on the half-strength MS medium at the presence of BA 0.6 mg/L. The rooted plantlets survived in greenhouse.

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Available abstract

A plant regeneration system from leaves of chrysanthemum was developed in this study. The optimum media were obtained for callus induction, shoot regeneration and rooting. Calli were induced from leaf explants cultured on the medium MS + BA 2 mg/L + NAA 0.2 mg/L. Bud shoots were induced from the calli cultured on the medium MS + BA 3 mg/L + NAA 0.1 mg/L, and rooted on the half-strength MS medium at the presence of BA 0.6 mg/L. The rooted plantlets survived in greenhouse.

Key concepts: Shoot, Explant culture, Callus, Regeneration (biology), Murashige and Skoog medium, Botany, Biology, Horticulture

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